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Image Search Results
Journal: iScience
Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells
doi: 10.1016/j.isci.2022.105402
Figure Lengend Snippet: RNA-seq and GO-analysis of FACS Sorted PURK-cocktail-treated cells at distinct differentiation time points (A) RNA-seq (HeatMap) showing the gene expression of PURK-cocktail-treated cells, compared to vehicle-treated control cells, on day-4 (D4) and day-7 (D7) of differentiation. PURK-cocktail treatment induced a genetic profile that closely resembled that of native cardiac Purkinje cells as compared to the control CMs. At day-4 (D4), the PURK-cocktail starts to induce a cardiac Purkinje-like genetic profile which becomes even more robust by day-7 (D7) of differentiation. Additionally, it can be observed that the expression of atrial or ventricular CM genes, as well as sinoatrial (SAN), atrioventricular (AVN) and His bundle-specific genes are down-regulated over time in the PURK-cocktail-treated cells, as the cells become more Purkinje-like. (B and C) GO-analyses showing over -expressed and down -expressed genes, respectively, on day-4 ( D4 ) of the differentiation of PURK-cocktail-treated cell. (D and E) GO-analyses showing the over -expressed and down -expressed genes, respectively, on day-7 ( D7 ) of the differentiation of PURK-cocktail-treated cells. The GO analyses collectively show the process of trans -differentiation from cardiomyocytes to cardiac Purkinje-like cells (PURK-cells).
Article Snippet:
Techniques: RNA Sequencing, Gene Expression, Control, Expressing
Journal: iScience
Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells
doi: 10.1016/j.isci.2022.105402
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Transfection, Knock-Out, Multiplex sample analysis, SYBR Green Assay, Reverse Transcription, Derivative Assay, Plasmid Preparation, Software
Journal: iScience
Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells
doi: 10.1016/j.isci.2022.105402
Figure Lengend Snippet: RNA-seq and GO-analysis of FACS Sorted PURK-cocktail-treated cells at distinct differentiation time points (A) RNA-seq (HeatMap) showing the gene expression of PURK-cocktail-treated cells, compared to vehicle-treated control cells, on day-4 (D4) and day-7 (D7) of differentiation. PURK-cocktail treatment induced a genetic profile that closely resembled that of native cardiac Purkinje cells as compared to the control CMs. At day-4 (D4), the PURK-cocktail starts to induce a cardiac Purkinje-like genetic profile which becomes even more robust by day-7 (D7) of differentiation. Additionally, it can be observed that the expression of atrial or ventricular CM genes, as well as sinoatrial (SAN), atrioventricular (AVN) and His bundle-specific genes are down-regulated over time in the PURK-cocktail-treated cells, as the cells become more Purkinje-like. (B and C) GO-analyses showing over -expressed and down -expressed genes, respectively, on day-4 ( D4 ) of the differentiation of PURK-cocktail-treated cell. (D and E) GO-analyses showing the over -expressed and down -expressed genes, respectively, on day-7 ( D7 ) of the differentiation of PURK-cocktail-treated cells. The GO analyses collectively show the process of trans -differentiation from cardiomyocytes to cardiac Purkinje-like cells (PURK-cells).
Article Snippet:
Techniques: RNA Sequencing, Gene Expression, Control, Expressing
Journal: iScience
Article Title: Direct reprogramming of cardiomyocytes into cardiac Purkinje-like cells
doi: 10.1016/j.isci.2022.105402
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Transfection, Knock-Out, Multiplex sample analysis, SYBR Green Assay, Reverse Transcription, Derivative Assay, Plasmid Preparation, Software